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After final departure preparations on both sides of the hatches (closed at ~1:30pm EDT), Discovery undocked this afternoon at 3:53pm from PMA-2 (Pressurized Mating Adapter 2) after a total docked time of 9d 20h 10m Discovery mass at undocking: 211,319 lbs (95,852 kg). [For undocking, the station was turned at ~2:39pm from -XVV through ~180 deg to +XVV ZLV (+x-axis in velocity vector, z-axis in local vertical, i.e., flying Shuttle in front again), put briefly on free drift for the undocking and then moded to 15A Stage attitude of +XVV TEA (Torque Equilibrium Attitude).]

Undocking was ~2 min prior to midnight (~3:56pm) to ensure good lighting during the subsequent flyaround (sunrise: ~9 min before flyaround start). After separation, Discovery completed the 360-deg station flyaround and obtained photo/video imagery of the ISS. The two separation burns were to be conducted at 5:08pm & 5:37pm.

KSC landing is nominally expected on 3/28 (Saturday) at ~1:43pm EDT. [If the landing occurs as planned, 15A mission duration will be 12d 17h 59m. Sandra Magnus’s total time in space will be 133d 16h 48m.]

For the biomed experiment INTEGRATED IMMUNE (Validating Procedures for Monitoring Crew member Immune Function), FE-2 Magnus conducted her final Liquid Saliva collections in the morning, and later assisted in drawing a blood sample from CDR Fincke. The Saliva Return Pouches and Blood Sleeves were then stored at ambient temperature on the Shuttle Middeck for return to ground. [IMMUNE protocol requires the collection to occur first thing post-sleep, before eating, drinking and brushing teeth, and all samples are stored at ambient temperature. Along with NUTRITION (Nutritional Status Assessment), INTEGRATED IMMUNE samples & analyzes participant’s blood, urine, and saliva before, during and after flight for changes related to functions like bone metabolism, oxidative damage and immune function to develop and validate an immune monitoring strategy consistent with operational flight requirements and constraints. The strategy uses both long an

d short duration crewmembers as study subjects.]

After the UPA DA (Urine Processor Assembly/Distillation Assembly) successfully completed its second operating run overnight, Wakata collected “snapshot” water samples from the WPA RIP (Water Processing Assembly/Rack Interface Panel), PWD (Potable Water Dispenser) Auxiliary Port & PWD Ambient Port, using the WPA common hose and afterwards transferring the sample bags to the Shuttle for return to Earth.

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