The Liverpool striker was banned for nine international matches and for four months from "all football activity", as well as receiving fine of 100,000 Swiss francs (?66,000), for the incident which took place in the final match of World Cup Group D.
"The Fifa appeal committee has decided to reject the appeals lodged by both the Uruguayan player Luis Suarez and the Uruguayan FA, and to confirm the decision rendered by the Fifa disciplinary committee on June 25 2014 in its entirety," said Fifa head of media Delia Fischer at a news conference in Rio de Janeiro.
"The terms of the decision taken by the Fifa appeal committee were communicated to the player and the Uruguayan FA today."
Both the player and the federation can make a further appeal to the Court of Arbitration for Sport.
Fischer added: "The relevant decision is not yet final and binding, i.e. an appeal to the Court of Arbitration of Sport is still possible by the player and/or the Uruguayan FA, subject to certain conditions."
